Innovating Neurological Research

ELISA (Enzyme-Linked Immunosorbent Assay)

ELISA is a classic and widely utilized immunoassay technique that detects and quantifies target proteins, peptides, or antibodies through specific antigen-antibody interactions. It employs an enzyme-conjugated detection antibody and a colorimetric, fluorescent, or chemiluminescent substrate to produce a measurable signal proportional to the analyte level. ELISA is known for its robustness, relative simplicity, and versatility across a wide range of applications, making it a staple in clinical diagnostics and research laboratories.

ECL (Electrochemiluminescence) Assay

Electrochemiluminescence (ECL) assays harness an electrical stimulus to excite a luminescent label on detection antibodies, generating a light signal that is directly proportional to the amount of target present. Platforms like MSD (Meso Scale Discovery) use this approach to offer high sensitivity, wide dynamic range, and low background noise. The electrochemiluminescent readout is particularly advantageous for multiplexing, allowing researchers to simultaneously measure multiple analytes in a single sample.

Simoa (Single Molecule Array)

Simoa technology revolutionizes immunoassays by enabling the detection of individual protein molecules through a digital readout system. This ultrasensitive approach traps individual beads—each capturing a single target molecule—within thousands of micro-wells and uses fluorescence to detect positive signals one bead at a time. As a result, Simoa delivers exceptionally low limits of detection and precise quantification for biomarkers, making it invaluable in translational research and early disease detection.

Olink PEA (Proximity Extension Assay)

Olink’s Proximity Extension Assay (PEA) leverages pairs of antibodies linked to unique DNA oligonucleotides, which hybridize only when the antibody pair binds in close proximity to the target protein. The resulting DNA duplex is amplified and quantified using real-time PCR or next-generation sequencing methods. This process enables highly specific and multiplexed protein measurements, providing detailed protein profiling in limited sample volumes with excellent sensitivity and specificity.